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1.
Nutrients ; 13(12)2021 Dec 11.
Article in English | MEDLINE | ID: mdl-34959987

ABSTRACT

Copious fluid intake is the most essential nutritional measure in the treatment of urolithiasis, and is suggested to be a protective factor in the primary prevention of urinary stone formation. Although the intake of black tea contributes to daily fluid intake, the high oxalate content could outweigh the beneficial effect of urine dilution. The present study investigated the effect of black tea consumption on urinary risk factors for kidney stone formation. Ten healthy men received a standardized diet for a period of ten days. Subjects consumed 1.5 L/day of fruit tea (0 mg/day oxalate) during the 5-day control phase, which was replaced by 1.5 L/day of black tea (86 mg/day oxalate) during the 5-day test phase. Fractional and 24-h urines were obtained. The intake of black tea did not significantly alter 24-h urinary oxalate excretion. Urinary citrate, an important inhibitor of calcium stone formation, increased significantly, while the relative supersaturation of calcium oxalate, uric acid, and struvite remained unchanged. No significantly increased risk for kidney stone formation could be derived from the ingestion of black tea in normal subjects. Further research is needed to evaluate the impact of black tea consumption in kidney stone patients with intestinal hyperabsorption of oxalate.


Subject(s)
Drinking/physiology , Eating/physiology , Kidney Calculi/prevention & control , Tea , Adult , Citric Acid/urine , Humans , Kidney Calculi/metabolism , Kidney Calculi/urine , Male , Oxalates/administration & dosage , Oxalates/analysis , Oxalates/metabolism , Risk Factors , Tea/chemistry , Time Factors , Uric Acid/metabolism , Young Adult
2.
Front Immunol ; 12: 617508, 2021.
Article in English | MEDLINE | ID: mdl-33732242

ABSTRACT

Diet has been associated with several metabolic diseases and may impact immunity. Increased consumption of meals with high oxalate content may stimulate urinary calcium oxalate (CaOx) crystals, which are precursors to CaOx kidney stones. We previously reported that CaOx stone formers have decreased monocyte cellular bioenergetics compared to healthy participants and oxalate reduces monocyte metabolism and redox status in vitro. The purpose of this study was to investigate whether dietary oxalate loading impacts monocyte cellular bioenergetics, mitochondrial complex activity, and inflammatory signaling in humans. Healthy participants (n = 40; 31.1 ± 1.3 years) with a BMI of 24.9 ± 0.6 kg/m2 consumed a controlled low oxalate diet for 3 days before drinking a blended preparation of fruits and vegetables containing a large amount of oxalate. Blood and urine were collected before (pre-oxalate) and for 5 h after the oxalate load to assess urinary oxalate levels, monocyte cellular bioenergetics and mitochondrial complex activity, and plasma cytokine/chemokine levels. Urinary oxalate levels significantly increased in post-oxalate samples compared to pre-oxalate samples. Monocyte cellular bioenergetics, mitochondrial complex I activity, and plasma cytokine and chemokine levels were altered to varying degrees within the study cohort. We demonstrate for the first time that dietary oxalate loading may impact monocyte metabolism and immune response in a cohort of healthy adults, but these response are variable. Further studies are warranted to understand oxalate mediated mechanisms on circulating monocytes and how this potentially influences CaOx kidney stone formation. Clinical Trial Registration: ClinicalTrials.gov, identifier NCT03877276.


Subject(s)
Dietary Supplements , Energy Metabolism/drug effects , Monocytes/drug effects , Monocytes/metabolism , Oxalates/administration & dosage , Signal Transduction/drug effects , Adult , Biomarkers , Electron Transport Chain Complex Proteins/metabolism , Female , Humans , Inflammation/etiology , Inflammation/metabolism , Inflammation/pathology , Leukocyte Count , Male , Mitochondria/drug effects , Mitochondria/metabolism , Urinalysis
3.
Benef Microbes ; 11(6): 547-559, 2020 Oct 12.
Article in English | MEDLINE | ID: mdl-33032472

ABSTRACT

Hyperoxaluria is a pathological condition which affects long-term health of kidneys. The present study evaluates the impact of the combination of Lactobacillus amylovorus SGL 14 and the plant extract Phyllantus niruri (namely Phyllantin 14™) on dietary hyperoxaluria. Safety and efficacy of Phyllantin 14 have been evaluated in vivo. Mice C57BL6 fed a high-oxalate diet were compared to mice fed the same diet administered with Phyllantin 14 by gavage for 6 weeks. Control mice were fed a standard diet without oxalate. No adverse effects were associated to Phyllantin 14 supplementation, supporting its safety. Mice fed a high-oxalate diet developed significant hyperoxaluria and those administered with Phyllantin 14 showed a reduced level of urinary oxalate and a lower oxalate-to-creatinine ratio. Soluble and insoluble caecal oxalate were significantly lower in treated group, a finding in agreement with the colonisation study, i.e. mice were colonised with SGL 14 after 3 weeks. Microbiota analysis demonstrated that both oxalate diet and Phyllantin 14 can differently modulate the microbiota. In conclusion, our findings suggest that Phyllantin 14 supplementation represents a potential supportive approach for reducing urinary oxalate and/or for enhancing the efficacy of existing treatments.


Subject(s)
Diet , Hyperoxaluria/therapy , Lactobacillus acidophilus , Oxalates/administration & dosage , Phyllanthus , Plant Extracts/therapeutic use , Animals , Bacterial Adhesion , Cecum/chemistry , Disease Models, Animal , Feces/chemistry , Gastrointestinal Microbiome , HT29 Cells , Humans , Hyperoxaluria/drug therapy , Hyperoxaluria/pathology , Kidney/pathology , Lactobacillus acidophilus/growth & development , Lactobacillus acidophilus/physiology , Male , Mice , Mice, Inbred C57BL , Oxalates/analysis , Oxalates/urine , Phytotherapy , Probiotics
4.
Nutrients ; 12(10)2020 Sep 24.
Article in English | MEDLINE | ID: mdl-32987890

ABSTRACT

Plant-based diets are associated with reduced risk of lifestyle-induced chronic diseases. The thousands of phytochemicals they contain are implicated in cellular-based mechanisms to promote antioxidant defense and reduce inflammation. While recommendations encourage the intake of fruits and vegetables, most people fall short of their target daily intake. Despite the need to increase plant-food consumption, there have been some concerns raised about whether they are beneficial because of the various 'anti-nutrient' compounds they contain. Some of these anti-nutrients that have been called into question included lectins, oxalates, goitrogens, phytoestrogens, phytates, and tannins. As a result, there may be select individuals with specific health conditions who elect to decrease their plant food intake despite potential benefits. The purpose of this narrative review is to examine the science of these 'anti-nutrients' and weigh the evidence of whether these compounds pose an actual health threat.


Subject(s)
Diet, Vegetarian , Nutrients , Phytochemicals/administration & dosage , Phytochemicals/adverse effects , Antioxidants/administration & dosage , Antioxidants/adverse effects , Antioxidants/analysis , Antithyroid Agents/administration & dosage , Antithyroid Agents/adverse effects , Antithyroid Agents/analysis , Cooking , Food Handling , Fruit/chemistry , Humans , Lectins/administration & dosage , Lectins/adverse effects , Lectins/analysis , Oxalates/administration & dosage , Oxalates/adverse effects , Oxalates/analysis , Phytic Acid/administration & dosage , Phytic Acid/adverse effects , Phytic Acid/analysis , Phytochemicals/analysis , Phytoestrogens/administration & dosage , Phytoestrogens/adverse effects , Phytoestrogens/analysis , Tannins/administration & dosage , Tannins/adverse effects , Tannins/analysis , Vegetables/chemistry
5.
Nutrients ; 12(9)2020 Sep 02.
Article in English | MEDLINE | ID: mdl-32887293

ABSTRACT

Oxalate is both a plant-derived molecule and a terminal toxic metabolite with no known physiological function in humans. It is predominantly eliminated by the kidneys through glomerular filtration and tubular secretion. Regardless of the cause, the increased load of dietary oxalate presented to the kidneys has been linked to different kidney-related conditions and injuries, including calcium oxalate nephrolithiasis, acute and chronic kidney disease. In this paper, we review the current literature on the association between dietary oxalate intake and kidney outcomes.


Subject(s)
Kidney/drug effects , Nephrolithiasis/diagnosis , Oxalates/administration & dosage , Oxalates/adverse effects , Animals , Diet , Disease Models, Animal , Glomerular Filtration Rate , Humans , Kidney/metabolism , Nephrolithiasis/etiology , Randomized Controlled Trials as Topic
6.
Eur J Pharmacol ; 882: 173261, 2020 Sep 05.
Article in English | MEDLINE | ID: mdl-32534073

ABSTRACT

Neuropeptide-Y (NPY) leads to angiogenesis and remodeling of the ischemic myocardium. The objective of this study is to assess the therapeutic potential of NPY in a model of acute myocardial ischemia using a nanoparticles delivery system targeted to tissue with oxidative stress. NPY3-36 was loaded onto copolyoxalate containing vanillyl alcohol (PVAX) using a double emulsification strategy. Adult C57BL/J6 mice (n = 49) were randomly divided into PVAX-NPY3-36 (n = 22), Vehicle (Saline) (n = 16), and Sham (n = 11) groups. The ischemia to left anterior descending artery was induced in PVAX-NPY3-36 or vehicle groups. The tissue was collected at the end of two weeks after assessing the functional and echocardiographic data. There was a significant decrease in infarction size and mortality in PVAX-NPY3-36 group compared to the Vehicle group (P = 0.01 and P = 0.05). On echocardiography, there was significant improvement in contractility and diastolic parameters (P = 0.01). On pressure-volume loop there was significant increase in stroke volume (P = 0.01), cardiac output (P = 0.01) and ventricular stroke work (P = 0.01) in the PVAX-NPY3-36 group. On Western blot analysis, there was a significant increase in pro-angiogenic factors Ang-1, TGF-ß, PDGF- ß and its receptors and VEGF in the ischemic tissue treated with PVAX-NPY3-36 as compared to Vehicle ischemic tissue (P = 0.01, P = 0.0003, and P < 0.05 respectively). It may be possible to have targeted delivery of labile neurotransmitters NPY3-36 to the ischemic myocardium using nanoparticle PVAX and achieving angiogenesis and significant functional improvement.


Subject(s)
Benzyl Alcohols/administration & dosage , Cardiotonic Agents/administration & dosage , Myocardial Ischemia/drug therapy , Nanoparticles/administration & dosage , Neovascularization, Physiologic/drug effects , Neuropeptide Y/administration & dosage , Oxalates/administration & dosage , Peptide Fragments/administration & dosage , Polymers/administration & dosage , Animals , Disease Models, Animal , Hemodynamics/drug effects , Male , Mice, Inbred C57BL , Myocardial Contraction/drug effects , Myocardial Ischemia/physiopathology , Ventricular Function, Left/drug effects
7.
J Am Soc Nephrol ; 30(10): 1857-1869, 2019 10.
Article in English | MEDLINE | ID: mdl-31296606

ABSTRACT

BACKGROUND: Serum oxalate levels suddenly increase with certain dietary exposures or ethylene glycol poisoning and are a well known cause of AKI. Established contributors to oxalate crystal-induced renal necroinflammation include the NACHT, LRR and PYD domains-containing protein-3 (NLRP3) inflammasome and mixed lineage kinase domain-like (MLKL) protein-dependent tubule necroptosis. These studies examined the role of a novel form of necrosis triggered by altered mitochondrial function. METHODS: To better understand the molecular pathophysiology of oxalate-induced AIK, we conducted in vitro studies in mouse and human kidney cells and in vivo studies in mice, including wild-type mice and knockout mice deficient in peptidylprolyl isomerase F (Ppif) or deficient in both Ppif and Mlkl. RESULTS: Crystals of calcium oxalate, monosodium urate, or calcium pyrophosphate dihydrate, as well as silica microparticles, triggered cell necrosis involving PPIF-dependent mitochondrial permeability transition. This process involves crystal phagocytosis, lysosomal cathepsin leakage, and increased release of reactive oxygen species. Mice with acute oxalosis displayed calcium oxalate crystals inside distal tubular epithelial cells associated with mitochondrial changes characteristic of mitochondrial permeability transition. Mice lacking Ppif or Mlkl or given an inhibitor of mitochondrial permeability transition displayed attenuated oxalate-induced AKI. Dual genetic deletion of Ppif and Mlkl or pharmaceutical inhibition of necroptosis was partially redundant, implying interlinked roles of these two pathways of regulated necrosis in acute oxalosis. Similarly, inhibition of mitochondrial permeability transition suppressed crystal-induced cell death in primary human tubular epithelial cells. PPIF and phosphorylated MLKL localized to injured tubules in diagnostic human kidney biopsies of oxalosis-related AKI. CONCLUSIONS: Mitochondrial permeability transition-related regulated necrosis and necroptosis both contribute to oxalate-induced AKI, identifying PPIF as a potential molecular target for renoprotective intervention.


Subject(s)
Acute Kidney Injury/pathology , Mitochondrial Transmembrane Permeability-Driven Necrosis , Necroptosis , Acute Kidney Injury/chemically induced , Animals , Cells, Cultured , Humans , Male , Mice , Oxalates/administration & dosage
8.
Can J Urol ; 25(6): 9585-9590, 2018 12.
Article in English | MEDLINE | ID: mdl-30553283

ABSTRACT

INTRODUCTION: Renal colic is commonly seen in the emergency department (ED), where the focus is on diagnosis and symptom control. Educational materials are sometimes provided upon discharge, however, no standard content has been established. We characterized the educational materials given to patients reporting to EDs in different regions across the U.S. for symptomatic kidney stones, specifically evaluating disease-specific information, symptom management, prevention strategies including dietary recommendations (DRs), and patient follow up plans. MATERIALS AND METHODS: Generic discharge instructions for patients presenting to EDs with renal colic were obtained from community hospitals and academic medical centers between October 2016 and November 2017. Hospitals were called directly. If the same discharge instructions were used by more than one hospital, each was included in our analysis. We assessed the different types of information provided with a focus on stone prevention and DRs by characterizing them into specific nutritional categories. RESULTS: Of 266 hospitals contacted, 79 provided discharge instructions. Of these, 51 (65%) provided some information on diet. While most recommended higher fluid intake, almost 40% endorsed unnecessary fluid restrictions. Recommendations to reduce protein and oxalate intake were common, but erroneous information for both was given. Nearly 1 in 5 EDs recommended lower calcium intake. Less than 30% of EDs mentioned that stones can have different composition or causes. Less than 30% referenced consultation with a registered dietitian nutritionist (RDN) or that dietary approaches to stone prevention are optimally individualized. Only 9 summaries recommended urologic follow up. CONCLUSIONS: Many ED discharge materials contain DRs for stone prevention. These recommendations can be inaccurate and/or inappropriate. Advice on diet and stone prevention is more appropriately addressed in the outpatient setting when more data (stone composition, serum and urine parameters) and expert consultants are available.


Subject(s)
Emergency Service, Hospital , Kidney Calculi/prevention & control , Patient Education as Topic/standards , Calcium/administration & dosage , Diet , Dietary Proteins/administration & dosage , Dietetics , Drinking , Humans , Kidney Calculi/therapy , Oxalates/administration & dosage , Patient Discharge , Referral and Consultation
9.
BMC Nephrol ; 19(1): 265, 2018 10 12.
Article in English | MEDLINE | ID: mdl-30314464

ABSTRACT

BACKGROUND: Ingestion of vitamin C is generally regarded as harmless. Oxalate nephropathy is an infrequent condition and is characterized by oxalate deposition in the renal tubules, in some cases resulting in acute kidney injury. It can be caused by overproduction of oxalate in genetic disorders and, more frequently, as a secondary phenomenon provoked by ingestion of oxalate or substances that can be transformed into oxalate in the patient. CASE PRESENTATION: We present a case of acute oxalate nephropathy in a 59-year-old black male with type 2 diabetes mellitus, who received a kidney transplant 11 years prior. He ingested a large amount of cashew pseudofruit ("cashew apple") during 1 month and developed acute kidney injury. His previous blood creatinine was 2.0 mg/dL, which increased to 7.2 mg/d; he required hemodialysis. He was subsequently discharged without need for dialysis; 3 months later his blood creatinine stabilized at 3.6 mg/dL. CONCLUSIONS: This pseudofruit is rich in ascorbic acid (vitamin C) and poor in oxalate. Urinary oxalate excretion begins to increase when amounts of ascorbic acid above bodily requirements are ingested, and may provoke acute oxalate nephropathy. The patient's oxalate acute nephropathy, in this case, was attributed to excessive vitamin C ingestion from the cashew pseudofruit associated with decreased renal function.


Subject(s)
Acute Kidney Injury/chemically induced , Acute Kidney Injury/surgery , Anacardium/adverse effects , Ascorbic Acid/adverse effects , Kidney Transplantation/trends , Oxalates/adverse effects , Acute Kidney Injury/diagnosis , Ascorbic Acid/administration & dosage , Humans , Male , Middle Aged , Oxalates/administration & dosage
10.
Int Urol Nephrol ; 50(9): 1583-1589, 2018 Sep.
Article in English | MEDLINE | ID: mdl-30039216

ABSTRACT

PURPOSE: The primary goal of this pilot study was to evaluate metabolic characteristics and to examine the impact of diet in patients with primary hyperoxaluria (PH) under controlled, standardized conditions. METHODS: Four patients with genetically confirmed PH collected 24 h urines on their habitual, self-selected diets and on day 1, 6, 7, 8, and 11 under controlled, standardized conditions. The [13C2]oxalate absorption, calcium, and ammonium chloride loading tests were performed. RESULTS: While none of the patients had abnormal findings from the calcium loading test, incomplete distal renal tubular acidosis (RTA) was diagnosed in each of the four patients. Dietary intervention resulted in a significant decrease in urinary oxalate expressed as molar creatinine ratio (mmol/mol) between 30 and 40% in two of four patients. The evaluation of dietary records revealed a high daily intake of oxalate-rich foods as well as gelatin-containing sweets and meat products, rich sources of hydroxyproline, under the habitual, self-selected diets of the two responders. Intestinal oxalate hyperabsorption of 12.4% in one of the two patients may have additionally contributed to the increased urinary oxalate excretion under the individual diet. CONCLUSIONS: Our pilot data indicate that patients with PH may benefit from a restriction of dietary oxalate and hydroxyproline intake. Further research is needed to define the role of distal RTA in PH and to evaluate the hypothesis of an acquired acidification defect.


Subject(s)
Hyperoxaluria, Primary/diet therapy , Hyperoxaluria, Primary/urine , Oxalates/administration & dosage , Oxalates/urine , Acidosis, Renal Tubular/diagnosis , Adolescent , Adult , Calcium/administration & dosage , Calcium/urine , Child , Creatinine/urine , Diet , Diet Records , Humans , Hydroxyproline/administration & dosage , Intestinal Absorption , Kidney Tubules, Distal , Male , Middle Aged , Pilot Projects
11.
Int J Dent Hyg ; 16(2): e79-e87, 2018 May.
Article in English | MEDLINE | ID: mdl-28925034

ABSTRACT

OBJECTIVES: The purpose of this study was to determine the efficacy of 3.14% dipotassium oxalate monohydrate-containing strip on the relief of dentinal hypersensitivity (DH). A second objective was to determine whether there was a difference in DH levels when the strip was self-applied vs applied by a dental professional. METHODS: Sixty subjects were randomized into self-applied, professionally applied or placebo-strip groups. Dentinal hypersensitivity was evaluated by a blinded examiner, using the Schiff Air Scale (SAS). A verbal rating scale (VRS) was also used to measure the subjects' perception of pain. Measures were taken at baseline, 30 minutes, 4 and 8 weeks post-treatment. RESULTS: There was a significant reduction in DH in all three groups (P ≤ .05) at 30 minutes and 8 weeks post-treatment. At the 4-week follow-up, only the self- and professionally applied (active ingredient) groups had a significant reduction in DH compared to baseline. When comparing the reduction in DH levels between groups, the only significant difference occurred between the professionally applied treatment group and the placebo group at the 4-week follow-up. There were no significant differences in DH reduction levels achieved between the self- and professionally applied groups (P > .05). CONCLUSIONS: This study confirmed the short-term (4 weeks) effectiveness of a single application of 3.14% dipotassium oxalate monohydrate-containing strip. Self- and professional application were not shown to be different.


Subject(s)
Dentin Desensitizing Agents/therapeutic use , Dentin Sensitivity/drug therapy , Oxalates/therapeutic use , Administration, Topical , Adult , Dentin Desensitizing Agents/administration & dosage , Double-Blind Method , Female , Humans , Male , Middle Aged , Minnesota , Oxalates/administration & dosage , Pain Measurement , Treatment Outcome
12.
Nutrients ; 9(5)2017 May 12.
Article in English | MEDLINE | ID: mdl-28498348

ABSTRACT

In chronic kidney disease (CKD), accumulation of uremic toxins is associated with an increased risk of death. Some uremic toxins are ingested with the diet, such as phosphate and star fruit-derived caramboxin. Others result from nutrient processing by gut microbiota, yielding precursors of uremic toxins or uremic toxins themselves. These nutrients include l-carnitine, choline/phosphatidylcholine, tryptophan and tyrosine, which are also sold over-the-counter as nutritional supplements. Physicians and patients alike should be aware that, in CKD patients, the use of these supplements may lead to potentially toxic effects. Unfortunately, most patients with CKD are not aware of their condition. Some of the dietary components may modify the gut microbiota, increasing the number of bacteria that process them to yield uremic toxins, such as trimethylamine N-Oxide (TMAO), p-cresyl sulfate, indoxyl sulfate and indole-3 acetic acid. Circulating levels of nutrient-derived uremic toxins are associated to increased risk of death and cardiovascular disease and there is evidence that this association may be causal. Future developments may include maneuvers to modify gut processing or absorption of these nutrients or derivatives to improve CKD patient outcomes.


Subject(s)
Gastrointestinal Microbiome , Micronutrients/toxicity , Renal Insufficiency, Chronic/microbiology , Cardiovascular Diseases/etiology , Cardiovascular Diseases/mortality , Carnitine/administration & dosage , Carnitine/toxicity , Choline/administration & dosage , Choline/toxicity , Diet , Humans , Methylamines/administration & dosage , Methylamines/toxicity , Micronutrients/administration & dosage , Oxalates/administration & dosage , Oxalates/toxicity , Phosphates/administration & dosage , Phosphates/toxicity , Phosphatidylcholines/administration & dosage , Phosphatidylcholines/toxicity , Tryptophan/administration & dosage , Tryptophan/toxicity , Tyrosine/administration & dosage , Tyrosine/toxicity
13.
Redox Biol ; 10: 211-220, 2016 12.
Article in English | MEDLINE | ID: mdl-27810736

ABSTRACT

Clinical and experimental observations indicate a critical role for vascular endothelial growth factor (VEGF), secreted by the retinal pigment epithelium (RPE), in pathological angiogenesis and the development of choroidal neovascularization (CNV) in age-related macular degeneration (AMD). RPE-mediated VEGF expression, leading to angiogenesis, is a major signaling mechanism underlying ocular neovascular disease. Inhibiting this signaling pathway with a therapeutic molecule is a promising anti-angiogenic strategy to treat this disease with potentially fewer side effects. Oxalomalate (OMA) is a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase (IDH), which plays an important role in cellular signaling pathways regulated by reactive oxygen species (ROS). Here, we have investigated the inhibitory effect of OMA on the expression of VEGF, and the associated underlying mechanism of action, using in vitro and in vivo RPE cell models of AMD. We found that OMA reduced the expression and secretion of VEGF in RPE cells, and consequently inhibited CNV formation. This function of OMA was linked to its capacity to activate the pVHL-mediated HIF-1α degradation in these cells, partly via a ROS-dependent ATM signaling axis, through inhibition of IDH enzymes. These findings reveal a novel role for OMA in inhibiting RPE-derived VEGF expression and angiogenesis, and suggest unique therapeutic strategies for treating pathological angiogenesis and AMD development.


Subject(s)
Angiogenesis Inhibitors/administration & dosage , Macular Degeneration/drug therapy , Oxalates/administration & dosage , Retinal Pigment Epithelium/drug effects , Vascular Endothelial Growth Factor A/genetics , Vascular Endothelial Growth Factor A/metabolism , Angiogenesis Inhibitors/pharmacology , Animals , Cell Line , Cell Movement/drug effects , Cell Proliferation/drug effects , Disease Models, Animal , Gene Expression Regulation/drug effects , Human Umbilical Vein Endothelial Cells , Humans , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Macular Degeneration/genetics , Macular Degeneration/metabolism , Mice , Oxalates/pharmacology , Reactive Oxygen Species/metabolism , Retinal Pigment Epithelium/metabolism , Signal Transduction/drug effects , Von Hippel-Lindau Tumor Suppressor Protein/metabolism
14.
Sci Rep ; 6: 30233, 2016 07 25.
Article in English | MEDLINE | ID: mdl-27452398

ABSTRACT

This study evaluated effect of oxalate on epithelial mesenchymal transition (EMT) and potential anti-fibrotic property of epigallocatechin-3-gallate (EGCG). MDCK renal tubular cells were incubated with 0.5 mM sodium oxalate for 24-h with/without 1-h pretreatment with 25 µM EGCG. Microscopic examination, immunoblotting and immunofluorescence staining revealed that oxalate-treated cells gained mesenchymal phenotypes by fibroblast-like morphological change and increasing expression of vimentin and fibronectin, while levels of epithelial markers (E-cadherin, occludin, cytokeratin and ZO-1) were decreased. EGCG pretreatment could prevent all these changes and molecular mechanisms underlying the prevention by EGCG were most likely due to reduced production of intracellular ROS through activation of Nrf2 signaling and increased catalase anti-oxidant enzyme. Knockdown of Nrf2 by small interfering RNA (siRNA) abrogated all the effects of EGCG, confirming that the EGCG protection against oxalate-induced EMT was mediated via Nrf2. Taken together, our data indicate that oxalate turned on EMT of renal tubular cells that could be prevented by EGCG via Nrf2 pathway. These findings also shed light onto development of novel therapeutics or preventive strategies of renal fibrosis in the future.


Subject(s)
Catechin/analogs & derivatives , Fibrosis/drug therapy , Kidney Tubules/drug effects , NF-E2-Related Factor 2/genetics , Animals , Catechin/administration & dosage , Dogs , Epithelial-Mesenchymal Transition/drug effects , Epithelial-Mesenchymal Transition/genetics , Fibrosis/genetics , Fibrosis/pathology , Humans , Kidney Tubules/pathology , Madin Darby Canine Kidney Cells , Oxalates/administration & dosage , RNA, Small Interfering/genetics , Signal Transduction/drug effects
15.
Appl Environ Microbiol ; 82(9): 2669-2675, 2016 May.
Article in English | MEDLINE | ID: mdl-26896138

ABSTRACT

Diet is one of the primary drivers that sculpts the form and function of the mammalian gut microbiota. However, the enormous taxonomic and metabolic diversity held within the gut microbiota makes it difficult to isolate specific diet-microbe interactions. The objective of the current study was to elucidate interactions between the gut microbiota of the mammalian herbivore Neotoma albigula and dietary oxalate, a plant secondary compound (PSC) degraded exclusively by the gut microbiota. We quantified oxalate degradation in N. albigula fed increasing amounts of oxalate over time and tracked the response of the fecal microbiota using high-throughput sequencing. The amount of oxalate degraded in vivo was linearly correlated with the amount of oxalate consumed. The addition of dietary oxalate was found to impact microbial species diversity by increasing the representation of certain taxa, some of which are known to be capable of degrading oxalate (e.g., Oxalobacter spp.). Furthermore, the relative abundances of 117 operational taxonomic units (OTU) exhibited a significant correlation with oxalate consumption. The results of this study indicate that dietary oxalate induces complex interactions within the gut microbiota that include an increase in the relative abundance of a community of bacteria that may contribute either directly or indirectly to oxalate degradation in mammalian herbivores.


Subject(s)
Diet , Gastrointestinal Microbiome/drug effects , Oxalates/administration & dosage , Sigmodontinae/microbiology , Animals , Bacteria/classification , Bacteria/genetics , Bacteria/metabolism , Biodiversity , Ecology , Feces/microbiology , Gastrointestinal Microbiome/genetics , Herbivory , Microbial Interactions , Oxalates/metabolism , Oxalobacter formigenes/drug effects , Oxalobacter formigenes/genetics , Oxalobacter formigenes/metabolism , Plant Extracts/administration & dosage
16.
Urolithiasis ; 44(1): 27-32, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26614109

ABSTRACT

Dietary modifications should be considered as a first line approach in the treatment of idiopathic calcium oxalate nephrolithiasis. The amounts of oxalate and calcium consumed in the diet are significant factors in the development of the disease due to their impact on urinary oxalate excretion. There are a number of strategies that can be employed to reduce oxalate excretion. The consumption of oxalate-rich foods should be avoided and calcium intake adjusted to 1000-1200 mg/day. To encourage compliance it should be emphasized to patients that they be vigilant with this diet as a deviation in any meal or snack could potentially result in significant stone growth. The evidence underlying these two modifications is outlined and other strategies to reduce urinary oxalate excretion are reviewed.


Subject(s)
Calcium Oxalate/metabolism , Kidney Calculi/prevention & control , Oxalates/urine , Calcium, Dietary/administration & dosage , Humans , Intestinal Absorption , Oxalates/administration & dosage , Oxalobacter formigenes/physiology
17.
Praxis (Bern 1994) ; 104(7): 353-9, 2015 Mar 25.
Article in French | MEDLINE | ID: mdl-25804778

ABSTRACT

Oxalate is a highly insoluble metabolic waste excreted by the kidneys. Disturbances of oxalate metabolism are encountered in enteric hyperoxaluria (secondary to malabsorption, gastric bypass or in case of insufficient Oxalobacter colonization), in hereditary hyperoxaluria and in intoxication (ethylene glycol, vitamin C). Hyperoxaluria causes a large spectrum of diseases, from isolated hyperoxaluria to kidney stones and nephrocalcinosis formation, eventually leading to kidney failure and systemic oxalosis with life-threatening deposits in vital organs. New causes of hyperoxaluria are arising recently, in particular after gastric bypass surgery, which requires regular and preemptive monitoring. The treatment of hyperoxaluria involves reduction in oxalate intake and increase in calcium intake. Optimal urine dilution and supplementation with inhibitors of kidney stone formation (citrate) are required. Some conditions may need vitamin B6 supplementation, and the addition of probiotics might be useful in the future. Primary care physicians should identify cases of recurrent calcium oxalate stones and severe hyperoxaluria. Further management of hyperoxaluria requires specialized care.


L'oxalate est un déchet métabolique peu soluble excrété par les reins, et les hyperoxaluries peuvent être distinguées en hyperoxaluries entériques, hyperoxaluries héréditaires et les intoxications (éthylène glycol, vitamine C). L'hyperoxalurie induit un large spectre de maladies allant de l'hyperoxalurie isolée, formation de calculs rénaux, voire d'une néphrocalcinose, à l'insuffisance rénale et l'oxalose systémique avec des dépôts s'accumulant dans de nombreux organes. De nouvelles causes d'hyperoxalurie sont apparues ces dernières années, en particulier les hyperoxaluries survenant à la suite d'un bypass gastrique. Le traitement des hyperoxaluries fait intervenir, d'une part, une diminution contrôlée des apports en oxalate et une augmentation des apports en calcium et, d'autre part, une dilution des urines et l'ajout d'inhibiteurs de la lithogenèse (citrate). Dans certaines conditions particulières, une supplémentation en vitamine B6 ou l'utilisation de probiotiques peuvent être envisagées. Le praticien doit rester attentif aux cas de calculs d'oxalate de calcium récidivants ou d'hyperoxalurie sévère et les adresser pour une prise en charge spécialisée et multidisciplinaire.


Subject(s)
Hyperoxaluria/diagnosis , Hyperoxaluria/etiology , Calcium/administration & dosage , Diagnosis, Differential , Humans , Hyperoxaluria/classification , Hyperoxaluria/complications , Hyperoxaluria, Primary/classification , Hyperoxaluria, Primary/complications , Hyperoxaluria, Primary/diagnosis , Hyperoxaluria, Primary/genetics , Intestine, Large/microbiology , Kidney Calculi/prevention & control , Kidney Calculi/urine , Oxalates/administration & dosage , Oxalates/urine , Oxalobacter formigenes/physiology , Risk Factors
19.
Urology ; 84(4): 779-81, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25102784

ABSTRACT

OBJECTIVE: To determine if fish oil supplementation reduces endogenous oxalate synthesis in healthy subjects. MATERIALS AND METHODS: Fifteen healthy non-stone-forming adults participated in this study. Subjects first abstained from using vitamins, medications, or foods enriched in omega-3 fatty acids for 30 days. Next, they collected two 24-hour urine specimens while consuming a self-selected diet. Subjects consumed an extremely low-oxalate and normal-calcium diet for 5 days and collected 24-hour urine specimens on the last 3 days of this diet. Next, the subjects took 2 fish oil capsules containing 650-mg eicosapentaenoic acid and 450-mg docosahexaenoic acid twice daily for 30 days. They consumed a self-selected diet on days 1-25 and the controlled diet on days 26-30. Twenty-four-hour urine samples were collected on days 28-30. Excretion levels of urinary analytes including oxalate and glycolate were analyzed. RESULTS: Although there was a significant reduction in urinary oxalate, magnesium, and potassium excretions and an increase in uric acid excretion during the controlled dietary phases compared with the self-selected diet, there were no significant differences in their excretion during controlled diet phases with and without fish oil supplementation. CONCLUSION: These results suggest that fish oil supplementation does not reduce endogenous oxalate synthesis or urinary oxalate excretion in normal adults during periods of extremely low oxalate intake. However, these results do not challenge the previously described reduction in urinary oxalate excretion demonstrated in normal subjects consuming a moderate amount of oxalate in conjunction with fish oil.


Subject(s)
Dietary Supplements , Fish Oils/administration & dosage , Oxalates/urine , Adult , Diet , Female , Humans , Male , Oxalates/administration & dosage
20.
J Transl Med ; 11: 306, 2013 Dec 12.
Article in English | MEDLINE | ID: mdl-24330782

ABSTRACT

BACKGROUND: Secondary hyperoxaluria either based on increased intestinal absorption of oxalate (enteric), or high oxalate intake (dietary), is a major risk factor of calcium oxalate urolithiasis. Oxalate-degrading bacteria might have beneficial effects on urinary oxalate excretion resulting from decreased intestinal oxalate concentration and absorption. METHODS: Twenty healthy subjects were studied initially while consuming a diet normal in oxalate. Study participants were then placed on a controlled oxalate-rich diet for a period of 6 weeks. Starting with week 2 of the oxalate-rich diet, participants received 2.6 g/day of a lactic acid bacteria preparation for 5 weeks. Finally, subjects were examined 4 weeks after treatment while consuming again a normal-oxalate diet. Participants provided weekly 24-hour urine specimens. Analyses of blood samples were performed before and at the end of treatment. RESULTS: Urinary oxalate excretion increased significantly from 0.354 ± 0.097 at baseline to 0.542 ± 0.163 mmol/24 h under the oxalate-rich diet and remained elevated until the end of treatment, as did relative supersaturation of calcium oxalate. Plasma oxalate concentration was significantly higher after 5 weeks of treatment compared to baseline. Four weeks after treatment, urinary oxalate excretion and relative supersaturation of calcium oxalate fell to reach initial values. CONCLUSIONS: Persistent dietary hyperoxaluria and increased plasma oxalate concentration can already be induced in healthy subjects without disorders of oxalate metabolism. The study preparation neither reduced urinary oxalate excretion nor plasma oxalate concentration. The preparation may be altered to select for lactic acid bacteria strains with the highest oxalate-degrading activity.


Subject(s)
Diet , Hyperoxaluria/therapy , Lactobacillus , Oxalates/administration & dosage , Probiotics , Chromatography, High Pressure Liquid , Female , Healthy Volunteers , Humans , Hyperoxaluria/etiology , Male
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